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本文对直视钳穿输精管结扎术、输精管注射粘堵术和可复性输精管注射栓堵术手术前后血清抗精子抗体的发生情况进行了连续观察并加以比较,客观评价了3种绝育术后免疫反应情况。采用间接免疫珠试验和浅盘凝集试验方法检测术前、术后3、6、12、24个月时血清中的抗精子抗体。结果:3种绝育方法术后3个月凝集抗体阳性率和滴度均明显高于术前,直视钳穿、粘堵、栓堵凝集抗体阳性率最高时分别为46.2％、42.2％和50.0％;用免疫珠试验检测3种绝育术后3个月的血清,均已有抗体产生,直视钳穿、粘堵、栓堵免疫珠抗体阳性率最高时分别为26.9％、38.5％和37.8％。经相关分析显示,用免疫珠试验和浅盘凝集试验检测3种术式的抗体阳性率间有良好的相关性。结果表明,输精管绝育术后可产生抗精子抗体,可复性输精管注射栓堵术后抗体阳性率稍高,其中术后12个月栓堵凝集抗体阳性率明显高于直视钳穿输精管结扎术,术后24个月栓堵免疫珠抗体阳性率明显高于粘堵。各术式免疫珠抗体以IgG为主。
In this paper, we observed and compared the occurrence of serum anti-sperm antibodies before and after surgery with direct pliers, vasectomy, vasectomy and retrograde vasojectile embolectomy, and objectively evaluated the effects of three kinds of sterilization Reaction situation. Anti-sperm antibodies in serum were detected by indirect immunobead and shallow plate agglutination test at 3, 6, 12 and 24 months after operation. Results: The positive rates and titers of agglutinating antibodies at 3 months after operation were significantly higher than those before operation (P <0.05). The positive rates of agglutination antibody were 46.2%, 42.2% and 50.0 %. Immuno-bead test detected the serum of three kinds of sterilization 3 months after the sterilization. All antibodies had been produced. The positive rate of antibodies was 26.9%, 38.5% and 37.8 %. Correlation analysis showed that there was a good correlation between the positive rate of three kinds of antibody detected by immuno bead test and shallow plate agglutination test. The results showed that anti-sperm antibodies could be produced after vasectomy, and the positive rate of antibody was slightly higher after retrofit vasoconstrictor injection and plugging, in which the positive rate of agglutination antibody blocking and embolism was significantly higher 12 months after surgery , And the positive rate of blocking antibody beads at 24 months after operation was significantly higher than that of viscous blockage. Various surgical immunoglobulin IgG-based.