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采用聚合酶链反应(PCR)对134例慢性前列腺炎病人的前列腺液进行沙眼衣原体DNA重复序列检测,结果阳性率为29.1%(39/134)。非慢性前列腺炎其他泌尿生殖道疾病患者的前列腺液15例作为对照,均未出现阳性反应。用PCR检测沙眼衣原体敏感性高(检测3.85pgDNA,在引物扩增片段517bp仍有可见的扩增信号),特异性强(与数种细菌、病毒、支原体、弓形虫均未出现沙眼衣原体待异性沉淀带),提示本法可用于临床诊断,在一定程度上替代培养技术,成为监测沙眼衣原体感染的实验室手段。
Polymerase chain reaction (PCR) of 134 cases of chronic prostatitis in patients with prostatic fluid C. trachomatis DNA sequence analysis, the positive rate was 29.1% (39/134). Non-chronic prostatitis other urogenital diseases in patients with prostatic fluid in 15 cases as a control, no positive reaction. Chlamydia trachomatis detection by PCR was highly sensitive (detection of 3.85 pg DNA, with a visible amplification signal at 517 bp in the primer amplified fragment) and strong specificity (no C. trachomatis to several bacteria, viruses, mycoplasmas, toxoplasma Heterosexual sedimentation zone), suggesting that this method can be used for clinical diagnosis, to some extent, alternative culture technology, become a laboratory means of monitoring Chlamydia trachomatis infection.