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用鹅膏覃氨酸(ibotenic acid)分3点注入SD鼠右侧尾壳核头部,制成Huntington舞蹈病模型。毁损后1个月,用胚龄15～17d同种胚鼠纹状体悬液植入模型鼠侧脑室。实验分正常对照组,模型对照组,单纯胚纹状体移植组(ST组),含基膜蛋白Laminin胚纹状体移植组(LST组)。ST组及LST组于移植后3和6个月的平均主动回避反应阳性率与模型组间均有显著差异;与正常组相比,LST组于移植后3和6个月时均无显著差异,而ST组至6月时方无显著差异。移植后6个月测试动物夜间活动次数,发现模型鼠活动过度,而ST组与LST组与正常鼠近似。动物在存活6个月测试后处死,脑切片观察发现,伤侧尾壳核萎缩,移植组织位于尾壳核背侧并突入侧脑室。LST组移植区大于ST组。两移植区均见ChAT、GABA及Leu-ENK免疫阳性细胞,其形态及大小与正常对照组尾壳核所见相似。LST组AChE阳性细胞突起较ST组多且长。本实验证明,用胚纹状体移植入Huntington舞蹈病模型鼠,移植物能存活、生长并发挥其功能效应;基膜蛋白能促进神经元突起生长。
The aorta chitin acid (ibotenic acid) was injected into the right caudal putamen of SD rats at three points to make the Huntington’s chorea model. One month after the injury, the lateral ventricle of the model mice was implanted with the suspension of the same embryonic rat striatum aged 15-17 days. The experimental group was divided into normal control group, model control group, simple embryo implantation group (ST group) and Laminin embryo striatum transplantation group (LST group). There was a significant difference between the model group and the average active avoidance reaction rate of ST group and LST group at 3 and 6 months after transplantation. Compared with the normal group, LST group had no significant difference at 3 and 6 months after transplantation , While there was no significant difference between ST group and June. Six months after transplantation, animals were tested for the number of night activities and found that the model rats were hyperactive, while the ST and LST groups were similar to the normal rats. The animals were sacrificed 6 months after their survival test. Brain slices revealed that the injured caudate putamen was atrophied. The transplanted tissue was located on the dorsal side of caudate putamen and protruded into the lateral ventricle. The LST group was larger than the ST group. ChAT, GABA and Leu-ENK immunopositive cells were found in the two transplantation areas, and their morphology and size were similar to that of the normal control group. The number of AChE positive cells in LST group was longer than that in ST group. This experiment demonstrated that the transplantation of embryonic striatum into Huntington’s chorea model mice can survive, grow and exert their functional effects; the basement membrane protein can promote the neurite outgrowth.