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目的确定脑内小胶质细胞是否表达甘露糖受体,以及在不同脑区甘露糖受体的表达是否存在差异,以进一步明确小胶质细胞的功能。方法 C57小鼠26只,分为侧脑室炎症模型组(10只)、全身炎症模型组(6只)和正常对照组(10只)。通过注射细菌脂多糖(LPS)建立全脑急性炎症模型,用免疫荧光双标技术对小鼠脑组织冷冻切片进行染色,激光共焦显微镜下观察小胶质细胞(Iba-1阳性)或激活小胶质细胞(OX-6阳性)与甘露糖受体(CD206)的共定位,比较皮层、海马、纹状体、黑质和小脑5个脑区小胶质细胞和CD206共定位比例的差异。结果正常小鼠和急性侧脑室炎症小鼠皮层内的部分小胶质细胞(包括Iba-1阳性细胞与OX-6阳性细胞)有CD206表达,CD206阳性小胶质细胞的比例分别是83%和94%;激活小胶质细胞(表达OX-6)与CD206有共定位,CD206阳性细胞中的激活小胶质细胞在皮层、海马、纹状体、黑质和小脑的比例分别是79%、81%、74%、53%和66%,而激活小胶质细胞中的CD206阳性细胞的比例则是63%、48%、49%、53%和52%。结论脑内静息和激活的小胶质细胞都能够表达甘露糖受体,表达量受炎症因素的影响并在不同脑区存在差异,其中黑质内CD206阳性细胞中的激活小胶质细胞的比例明显低于其他脑区;激活小胶质细胞中CD206阳性细胞的比例则在海马偏低。
Objective To determine whether microglia in the brain express mannose receptor and whether there are differences in mannose receptor expression in different brain regions to further clarify the function of microglia. Methods Twenty-six C57 mice were divided into four groups: model group with lateral ventricle inflammation (10 rats), model group with systemic inflammation (6 rats) and normal control group (n = 10). The whole brain acute inflammation model was established by injecting bacterial lipopolysaccharide (LPS). The frozen section of mouse brain was stained with double immunofluorescence technique. The microglial cells (Iba-1 positive) or small activation Colocalization of glial cells (OX-6 positive) with mannose receptor (CD206) was performed to compare the co-localization of microglia and CD206 in the cortex, hippocampus, striatum, substantia nigra and cerebellum. Results Some microglia (including Iba-1 positive cells and OX-6 positive cells) in normal mouse and acute lateral ventricle mice had CD206 expression, and the percentage of CD206 positive microglial cells was 83% and 94%. Activated microglia (expressing OX-6) co-localized with CD206. The percentage of activated microglial cells in CD206 positive cells in the cortex, hippocampus, striatum, substantia nigra and cerebellum were 79% 81%, 74%, 53%, and 66%, while the percentage of activated CD206 positive cells in microglia was 63%, 48%, 49%, 53%, and 52%, respectively. Conclusion Both microglia resting and activated in the brain can express mannose receptor, the expression level is affected by inflammatory factors and there are differences in different brain regions. Among microglial cells in substantia nigra on which CD206 positive cells activate microglia The proportion was significantly lower than other brain regions; activated microglial cells in the proportion of CD206-positive in the lower hippocampus.