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作者以夜猴为模型,研究了在使用完全福氏佐剂时,恶性疟原虫裂殖子表面蛋白C末端42kDa片段(BVp42)的免疫原性和保护效果;并探讨了接种动物所产生的抗体对恶性疟原虫体外生长的抑制以及与体内抗疟保护的关系。 将8只未接触恶性疟原虫的健康夜猴随机平均分为对照组和实验组,分别用CFA和CFA+BVp42进行四次免疫,每次间隔时间为21天。每次免疫后的第7天和14天采3ml血进行ELISA、IFA的效价测定和抗体应答动力学分析。实验组动物的抗体效价随免疫过程不断升高,在第四次免疫后达到最高值并趋稳定,ELISA和IFA效价均分别在1:10~6和1:640O以上,而对照组夜猴呈阴性反应或效价很低。实验组动物产生的抗体与虫源性MSP-1、酵母重组MSP-1 C末端
The authors studied the immunogenicity and protective effect of a 42 kDa fragment of the C-terminal end of Plasmodium falciparum merozoite surface protein (BVp42) using a full-fledged monkey as a model, and investigated the effects of vaccinated animals on antibodies Inhibition of Plasmodium falciparum in vitro growth and its relationship with protection against malaria in vivo. Eight healthy non-infected macaques were randomly divided into control and experimental groups, and were immunized four times with CFA and CFA + BVp42 at intervals of 21 days. ELISA was performed on 3 and 7 days after each immunization with ELISA, IFA titer and antibody response kinetics. The antibody titers of the experimental group increased with the course of the immune process, reaching the highest value and stabilizing after the fourth immunization. The titer of the ELISA and IFA were above 1: 10 ~ 6 and 1: 640O, respectively, while that of the control group Negative reaction or low titer. Antibodies produced by the animals in the experimental group were co-cultured with insect-derived MSP-1, yeast C-terminal MSP-1