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目的探讨采用分子印迹技术合成罗红霉素分子印迹聚合物的方法,并对其特性进行研究。方法以罗红霉素为模板分子,α-甲基丙烯酸为功能单体,以乙二醇二甲基丙烯酸酯为交联剂,采用分子印迹技术合成分子印迹聚合物。通过特异性吸附实验研究分子印迹聚合物的特性。结果通过静态平衡吸附法研究了模板聚合物的结合动力学以及该聚合物的结合能力和选择特性,通过Scatchard分析法研究了印迹聚合物对模板分子的结合特性,经计算聚合物的最大表观结合量(Qmax)和平衡离解常数(Kd)分别为90.3 mg.g-1和1.35 mg.mL-1。结论合成的分子印迹聚合物有形状、大小以及识别位点都与模板分子相匹配的空穴,即具有印迹作用。
Objective To investigate the molecular imprinting technology for synthesis of roxithromycin molecularly imprinted polymers and to study their properties. Methods The molecularly imprinted polymers were synthesized by molecular imprinting using roxithromycin as template and α-methacrylic acid as functional monomer and ethylene glycol dimethacrylate as crosslinking agent. The characteristics of molecularly imprinted polymers were studied by specific adsorption experiments. Results The binding kinetics of the template polymer and the binding ability and selectivity of the polymer were studied by static equilibrium adsorption. The binding properties of the imprinted polymer to the template were studied by Scatchard analysis. The maximum apparent polymer The binding capacity (Qmax) and equilibrium dissociation constant (Kd) were 90.3 mg.g-1 and 1.35 mg.mL-1, respectively. Conclusion The synthesized molecularly imprinted polymer has the shape, size and holes that match the template molecule with the recognition site, that is, the imprinting effect.