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目的：从分子水平观察肝细胞癌组织ＨＢＶＤＮＡ和癌基因的表达情况及与发病的关系。方法：应用原位核酸分子杂交技术研究４５例原发性肝细胞癌和２５例肝硬化的石蜡切片组织中ＨＢＶＤＮＡ和ｎ－ｒａｓ、ｃ－ｍｙｃ癌基因表达情况。结果：在肝细胞癌中见有ｎ－ｒａｓ和ｃ－ｍｙｃ表达增强，其检出率分别为６８．９％（３１／４５）和４０．０％（１８／４５），高于肝硬化组（２８．０％，８．０％）（Ｐ＜０．０５）。４０％肝细胞癌病例（１８／４５）两种癌基因同时过量表达，４５例肝细胞癌组织中有１９例存在有ＨＢＶＤＮＡ序列（４２．２％）并均具有ｎ－ｒａｓ或ｃ－ｍｙｃ过度表达。结论：两种癌基因ＨＢＶＤＮＡ可能有激活癌基因作用，肝细胞癌发生中可能起着重要的协同作用。ＨＢＶ感染与肝细胞癌发生有密切关系。
Objective: To observe the expression of HBVDNA and oncogene in hepatocellular carcinoma and its relationship with pathogenesis. Methods: The expression of HBVDNA, n-ras and c-myc oncogenes in 45 cases of hepatocellular carcinoma and 25 cases of liver cirrhosis were studied by in situ nucleic acid hybridization. Results: The expression of n-ras and c-myc was increased in hepatocellular carcinoma. The detection rates were 68.9% (31/45) and 40.0% (18/45), respectively, higher than those of cirrhosis group. (28.0%, 8.0%) (P<0.05). In the case of 40% hepatocellular carcinoma (18/45), two oncogenes were simultaneously overexpressed. In the 45 cases of hepatocellular carcinoma, 19 cases had HBV DNA sequence (42.2%) and all had n-ras or c-myc excess. expression. Conclusion: The two oncogene HBVDNA may activate oncogenes, which may play an important synergistic role in the occurrence of hepatocellular carcinoma. HBV infection is closely related to the occurrence of hepatocellular carcinoma.