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以两种不同基因型冬小麦种子为试验材料,研究了外源Ca(NO3)2和CaC l2对小麦萌发过程中盐害的缓解作用。结果表明,150mmol L-1NaC l胁迫明显抑制小麦种子的萌发。盐胁迫主要影响小麦根和芽的伸长,对根数的影响较小。种子萌发过程中,小偃6号的耐盐性优于陕229。外源钙对两种小麦盐胁迫效应的缓解作用不同。6mmol L-1钙离子可明显减轻陕229的盐害作用,对小偃6号作用不大。盐胁迫对种子萌发过程中的蛋白酶和淀粉酶活性具有明显的抑制作用。盐胁迫下,小偃6号种子的蛋白酶和淀粉酶活性明显高于陕229。钙离子对盐胁迫下小麦萌发过程胚乳内蛋白酶的活性影响较大,对淀粉酶活性影响较小。
The effects of exogenous Ca (NO3) 2 and CaCl2 on the salt damage of wheat during germination were studied using two different wheat genotypes as tested materials. The results showed that 150mmol L-1NaC l significantly inhibited the germination of wheat seeds. Salt stress mainly affected the elongation of wheat roots and shoots and had less effect on root number. Seed germination process, Xiaoyan 6 salt tolerance is better than Shaan 229. Exogenous calcium has a different alleviating effect on salt stress of two kinds of wheat. 6mmol L-1Ca2 + can significantly reduce the salt damage effect of Shaan 229 and little effect on Xiaoyan 6. Salt stress significantly inhibited the activity of protease and amylase during seed germination. Under salt stress, the protease and amylase activities of Xiaoyan 6 seeds were significantly higher than that of Shaan 229. The effect of Ca2 + on the activity of protease in the endosperm of wheat under salt stress was greater than that on the activity of amylase.