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目的克隆家兔泛素样小分子修饰因子-2(SUMO-2)基因,并预测其蛋白质结构和功能。方法应用分子克隆技术从家兔肝组织中克隆SUMO-2基因,用DNAMAN、CBS等生物软件及网络分析平台分析克隆所得到的SUMO-2基因序列及其编码的蛋白结构。结果测序结果显示成功克隆家兔SUMO-2基因片段,序列分析显示氨基酸序列与其他物种源性SUMO-2同源性较高,家兔SUMO-2蛋白氨基酸序列相对分子质量为10 826.9,等电点为4.70,蛋白主要以无规则卷曲形式存在(56.84%),可能含有1个N-糖基化位点,2个N-豆蔻酰化位点,1个丝氨酸磷酸化位点,2个苏氨酸磷酸化位点及2个蛋白激酶C磷酸化位点。无信号肽区域,无跨膜区,该蛋白可能为可溶性蛋白。结论成功克隆家兔SUMO-2基因,为进一步研究SUMO-2与肝纤维化的关系及其功能奠定基础。
Objective To clone rabbit SUMO-2 gene and predict its protein structure and function. Methods The SUMO-2 gene was cloned from rabbit hepatic tissue by molecular cloning technique. The SUMO-2 gene sequence and the protein structure of the cloned SUMO-2 gene were analyzed by using biological software such as DNAMAN, CBS and network analysis platform. Results Sequencing results showed that the rabbit SUMO-2 gene fragment was successfully cloned. Sequence analysis showed that the amino acid sequence shared high homology with SUMO-2 derived from other species. The amino acid sequence of SUMO-2 protein in rabbits was 10 826.9, Point was 4.70. The protein mainly existed in irregular curly form (56.84%), which may contain one N-glycosylation site, two N-myristoylation sites, one serine phosphorylation site, two Amino acid phosphorylation sites and two protein kinase C phosphorylation sites. No signal peptide region, no transmembrane region, the protein may be soluble protein. Conclusion The rabbit SUMO-2 gene was cloned successfully, which laid a foundation of further study on the relationship between SUMO-2 and liver fibrosis and its function.