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为观察钙离子拮抗剂对环氧酶 2 (COX 2 )表达的影响及探讨COX 2在缺血损伤过程中的作用 ,应用免疫组化方法 ,观察脑缺血模型大鼠缺血和尼莫通干预时环氧酶 2蛋白的表达。结果 :2组的假手术亚组、缺血 1h和 4h亚组均无阳性染色细胞 ,缺血 1 2h后 ,开始出现阳性染色细胞。尼莫通组的阳性着色细胞数显著高于单纯缺血组 ,分别为 :缺血 1 2h 56± 1 1 /mm2 与 4 5± 1 8/mm2 (P <0 .0 5) ;缺血 2 4h 1 1 7± 1 6/mm2 与 69± 1 4 /mm2 (P <0 .0 1 ) ;缺血 4 8h 81± 1 4 /mm2 与 4 1± 1 3/mm2 (P <0 .0 1 )。阳性染色仅见于梗死周围区形态完整的神经元。尼莫通组的脑梗死面积[( 2 8.30 8± 3.959)mm2 ]显著小于对照组 [( 46.780± 6.1 67)mm2 ,P <0 .0 1 ]。提示 :钙离子拮抗剂可有效减轻缺血性脑损伤 ,在梗死周围区COX 2延迟表达以及钙离子拮抗剂增加其表达 ,提示COX 2存在抗缺血损伤的作用
To observe the effect of calcium antagonists on the expression of cyclooxygenase 2 (COX 2) and the role of COX 2 in ischemic injury, immunohistochemistry was used to observe the changes of ischemia and nimoton Interfering expression of cyclooxygenase 2 protein. Results: There were no positive staining cells in subgroups of sham operation, ischemic 1h and 4h groups. After 12h of ischemia, positive staining cells began to appear. The number of positive cells in the nimotong group was significantly higher than that in the ischemia group, respectively: ischemia 12 h 56 ± 1 1 / mm2 and 4 5 ± 1 8 / mm2 (P <0.05); ischemia 2 4h 11 7 ± 1 6 / mm 2 and 69 ± 1 4 / mm 2 (P 0 01); ischemia 4 8h 81 ± 14 / mm 2 and 4 1 ± 1 3 / mm 2 (P 0 01 ). Positive staining was found only in the infarcted area around the integrity of neurons. The infarct size in the nimotop group was significantly (2 8.30 8 ± 3.959) mm 2 less than that in the control group [(46.780 ± 6.1 67) mm 2, P 0 01). Tip: Calcium antagonists can effectively reduce ischemic brain injury, delayed expression of COX 2 in the peri-infarct region and increased expression of calcium antagonists, suggesting that COX 2 has an anti-ischemic effect