论文部分内容阅读
目的:探讨肿瘤患者末梢血多形核白细胞(PMN)活性氧(ROS)强度和超氧化物歧化酶(SOD) 水平与肿瘤发生、发展的关系。方法:采用流式细胞仪法及邻苯三酚自氧化法对60例肿瘤患者及60例健康体检者末梢血PMN的ROS强度、SOD活性进行检测。结果:PMN的活性氧产生肿瘤组[(387．12±2．87)MFI] 明显高于对照组[(112．34±3．64)MFI](P<0．01),原发组[(493．56±3．44)MFI]高于转移组[(269．42± 3．66)MFI] (P<0．01);SOD水平肿瘤组[(218．80±4．59)U·g-1 Hb]明显低于对照组[(320．57± 3．35)U·g-1 Hb] (P<0．05),原发组[(189．4±2．85)U·g-1 Hb]低于转移组[(256．13± 3．66)U·g-1 Hb](P<0．05)。结论:肿瘤患者存在活性氧代谢失衡,且活性氧代谢情况可能与肿瘤的不同发展阶段有关。
Objective: To investigate the relationship between the level of reactive oxygen species (ROS) and the level of superoxide dismutase (SOD) in peripheral blood polymorphonuclear leukocytes (PMN) and the occurrence and development of tumor in patients with cancer. Methods: Flow cytometry and pyrogallol autoxidation were used to detect the level of ROS and the activity of SOD in peripheral blood PMN of 60 cancer patients and 60 healthy volunteers. Results: The MFI of PMN in tumor group [(387.12 ± 2.87) MFI] was significantly higher than that in control group [(112.34 ± 3.64) MFI] (P <0.01) (493.56 ± 3.44) MFI] was higher than that in the metastasis group [(269.42 ± 3.66) MFI] (P <0.01) · G-1 Hb] was significantly lower than that of the control group [(320.57 ± 3.35) U · g-1 Hb] (P <0.05) · G-1 Hb] was lower than that in the metastasis group [(256.13 ± 3.66) U · g-1 Hb] (P <0.05). CONCLUSION: There is an imbalance of reactive oxygen species metabolism in tumor patients, and the condition of reactive oxygen metabolism may be related to different stages of tumor development.