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目的:通过对比颈总动脉套管法与颈外动脉结扎法建立动脉粥样硬化兔模型的试验研究,比较2种方法在制模时间,模型稳定性方面的差异。方法:雄性新西兰兔30只,随机分成空白组(n=6);套管组(n=8);结扎组(n=8);高脂组(n=8)。所有试验兔在高脂喂养前、后分别检测血脂水平,于第8周处死动物,取套管近心端与结扎近心端血管行病理学检查。结果:高脂组饲养8周形成明显的高脂血症;与高脂组相比,单纯套管法颈动脉内膜脂质沉积较轻,但内膜明显增厚,内膜、中膜、外膜均出现不同程度的坏死;结扎法病变比套管法轻,但仍严重于正常组。结论:与单纯高脂饲养法相比,单纯套管法试验兔8周也可形成明显的动脉粥样性血管病变,套管近心端可形成局部低切应力模型,模型稳定性好。
OBJECTIVE: To establish an experimental model of atherosclerosis in rabbits by comparing the method of carotid artery cannulation and external carotid artery ligation, and to compare the differences of the two methods in modeling time and model stability. Methods: Thirty male New Zealand rabbits were randomly divided into blank group (n = 6), cannula group (n = 8), ligation group (n = 8) and hyperlipidemia group (n = 8). All rabbits were tested for serum lipid levels before and after high-fat diet, animals were sacrificed on the eighth week, and the proximal end of the cannula and the proximal end of the ligated blood vessels were examined by pathology. Results: Hyperlipidemia was found in the high fat diet for 8 weeks. Compared with the high fat diet, the carotid artery lipid deposition in the simple cannula was lighter, but the intima was thicker. The intima, Outer membrane showed varying degrees of necrosis; ligation lesion was lighter than the cannula, but still serious in the normal group. CONCLUSION: Compared with simple hyperlipidemic feeding method, pure cannula method can also induce obvious atherosclerotic vascular lesions in rabbits for 8 weeks. Local low-shear stress model can be formed in the proximal end of the cannula and the model has good stability.