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目的评价谷胱甘肽(GSH)对顺铂(C-DDP)引起的肾毒性的影响。方法采用随机分组法对30只Wistar大鼠分成对照组、C-DDP组和C-DDP+GSH组。对照组给予生理盐水(20ml/kg,腹腔注射,每周2次,共5周),C-DDP组大鼠给予C-DDP(2mg/kg,腹腔注射,每周2次,共5周);C-DDP+GSH组除与C-DDP组给药相同外,另同时给予GSH(0.3ml/100g,腹腔注射)。5周后将大鼠处死。取肾脏进行病理检查及用电感耦合等离子体原子发射光谱(ICP-AES)法测定肾脏中铂的含量。结果肾组织中铂浓度结果显示,C-DDP组与C-DDP+GSH组铂浓度的差异没有统计学意义,说明GSH不影响C-DDP在肾脏中的蓄积量。肾脏病理组织学结果表明,C-DDP可引起肾脏组织学改变,而GSH可减轻C-DDP所致的肾损伤,说明GSH对C-DDP引起的肾损伤在病理上有一定程度的缓解。结论GSH在不影响C-DDP蓄积量的情况下,一定程度上可以中和C-DDP引起的肾毒性。
Objective To evaluate the effect of glutathione (GSH) on nephrotoxicity induced by cisplatin (C-DDP). Methods 30 Wistar rats were randomly divided into control group, C-DDP group and C-DDP + GSH group. Rats in C-DDP group were treated with C-DDP (2mg / kg, intraperitoneal injection twice a week for 5 weeks) with saline (20ml / kg, intraperitoneal injection twice a week for 5 weeks) . In C-DDP + GSH group, GSH (0.3ml / 100g, intraperitoneal injection) was administered simultaneously with C-DDP group. Rats were sacrificed after 5 weeks. The kidneys were taken for pathological examination and the content of platinum in kidneys was determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). Results The concentration of platinum in renal tissue showed no significant difference in platinum concentration between C-DDP group and C-DDP + GSH group, indicating that GSH did not affect the accumulation of C-DDP in the kidney. Renal histopathology results showed that C-DDP can cause histological changes in the kidney, and GSH can reduce renal damage caused by C-DDP, indicating that GSH on C-DDP-induced renal injury in the pathological extent to some extent. Conclusion GSH can neutralize the nephrotoxicity induced by C-DDP to a certain extent without affecting the accumulation of C-DDP.