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单克隆抗体技术问世以来,应用免疫学技术测定细胞表面蛋白的方法就显得更加重要了。本文介绍一种新型、简易、准确,并能快速地对细胞表面标记物进行半定量的间接血细胞凝集法。并可进行淋巴细胞亚群签别和筛选杂交瘤的细胞株。方法:一、制备被检测的细胞。T 淋巴细胞系Molt 3和Jurkat 在含2mML-谷氨酰胺,10%小牛血清(FCS)50/μg/ml 庆大霉素的RPMI 1640培养基中在37℃,湿压为95%,含5% CO_2的情况下悬浮培养。用
Since the advent of monoclonal antibody technology, it has become even more important to use immunological techniques to determine cell surface proteins. This article presents a new, easy, accurate and rapid method of semi-quantitation of cell surface markers by indirect hemagglutination. And lymphocyte subsets can sign and screening hybridoma cell lines. Methods: First, prepare the cells to be tested. The T lymphocyte lines Molt 3 and Jurkat were wet at 95% in RPMI 1640 medium containing 2 mM L-glutamine, 10% fetal bovine serum (FCS) 50 / μg / ml gentamicin at 37 ° C, 5% CO2 in the case of suspension culture. use