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目的观察复方瑞康欣对吗啡依赖戒断大鼠焦虑行为的影响,并探讨其作用机制。方法健康雄性SD大鼠,剂量递增法sc吗啡10 d,确认吗啡依赖模型建立成功后,在吗啡自然戒断的1~3 d,复方瑞康欣100、200、300mg/kg和丁螺环酮15 mg/kg ig治疗(2次/d),于末次吗啡注射后72 h,高架十字迷宫实验评价大鼠的焦虑水平,免疫组化检测各组大鼠海马突触素的表达。结果与生理盐水(NS)治疗组比较,复方瑞康欣200、300 mg/kg和丁螺环酮治疗能明显增加大鼠进入开臂次数和时间的比例(P<0.01、0.05),同时海马突触素的表达显著降低(P<0.01)。结论复方瑞康欣对吗啡依赖大鼠戒断后的焦虑治疗效果明显;逆转焦虑大鼠海马增高的突触素/突触,可能是复方瑞康欣临床治疗吗啡戒断后焦虑的机制之一。
Objective To observe the effect of compound Ruikangxin on anxiety of morphine-dependent withdrawal rats and explore its mechanism. METHODS: Male Sprague-Dawley rats were dose-escalated with morphine for 10 days to confirm that the morphine-dependent model was successfully established. After 1-3 days of natural morphine withdrawal, the compound Ruikangxin 100, 200, 300 mg/kg and buspirone At 15 mg/kg ig treatment (2 times daily), 72 hours after the last morphine injection, the rat’s anxiety level was evaluated by an elevated plus-maze test. The expression of synaptophysin in hippocampus was detected by immunohistochemistry. Results Compared with saline (NS) treatment group, the compound Ruikangxin 200, 300 mg/kg and buspirone treatment significantly increased the proportion of rats entering the open arms and time (P<0.01, 0.05), while the hippocampus Synaptophysin expression was significantly reduced (P<0.01). Conclusion Compound Ruikangxin has significant effect on anxiety after morphine withdrawal in rats. Reversing the increased synaptophysin/synapses in the hippocampus of anxious rats may be one of the mechanisms of compound Ruikangxin’s clinical treatment of anxiety after morphine withdrawal. .