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目的:建立测定健康受试者血浆样品中佐米曲普坦浓度的液相色谱-质谱(HPLC-MS)定量分析方法。方法:血浆样品经乙腈直接沉淀并在真空泵中挥干后,利用 HPLC-MS/MS 选择性离子检测。结果:本法线性范围为0.1～40ng·mL~(-1),最小检出浓度为0.05 ng·mL~(-1),日内、日间 RSD 均小于15%。结论:本法可以用于测定血浆中佐米曲普坦浓度,方法准确、灵敏、快速,重现性好。
OBJECTIVE: To establish a liquid chromatography-mass spectrometry (HPLC-MS) quantitative method for the determination of zolmitriptan in plasma of healthy subjects. Methods: Plasma samples were directly precipitated with acetonitrile and evaporated in a vacuum pump before being detected by HPLC-MS / MS selective ionization. Results: The linear range of this method was 0.1 ~ 40 ng · mL ~ (-1) and the minimum detectable concentration was 0.05 ng · mL ~ (-1). The intra-day and inter-day RSD were less than 15%. Conclusion: This method can be used to determine the concentration of zolmitriptan in plasma. The method is accurate, sensitive, rapid and reproducible.