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目的研究慢性乙型肝炎(CHB)患者外周血IL-7含量与T淋巴细胞亚群、调节性T细胞百分比的关系,探究IL-7对慢性乙型肝炎细胞免疫作用的影响。方法慢性乙型肝炎患者组、慢性乙型肝炎病毒(HBV)携带组、健康对照组,采用ELISA法检测受试者外周血IL-7含量,流式细胞仪技术检测外周血T淋巴细胞亚群和CD4+CD25+调节性T细胞百分比。结果 CHB组、HBV携带组、健康对照组外周血IL-7含量分别为(1.68±0.58)ng/mL、(3.83±0.62)ng/mL和(6.25±0.32)ng/mL,3组间存在显著性差异(P<0.05);CHB组、HBV携带组、健康对照组外周血T细胞亚群CD4+T淋巴细胞分别为(31.3±3.3)%、(38.1±6.8)%、(43.2±9.4)%,CD8+T淋巴细胞分别为(24.8±5.2)%、(28.1±3.4)%、(30.5±8.6)%;CD4+CD25+调节性T细胞分别为(10.9±1.3)%、(7.4±1.0)%、(4.2±1.1)%。CHB组外周血IL-7明显低于HBV携带组及健康对照组,且与CD4+T细胞百分比呈负相关(r1=-0.58,P<0.05),与CD8+T细胞百分比呈正相关(r2=0.63,P<0.05),与CD4+CD25+调节性T细胞百分比呈负相关(r3=-0.43,P<0.05)。结论 CHB患者外周血IL-7负调控调节性T细胞,对CD8+T细胞的细胞免疫有促进作用。
Objective To investigate the relationship between the level of IL-7 in peripheral blood and the percentage of T lymphocyte subsets and regulatory T cells in patients with chronic hepatitis B (CHB) and explore the effect of IL-7 on the cellular immunity of chronic hepatitis B patients. Methods The levels of IL-7 in peripheral blood of patients with chronic hepatitis B, chronic hepatitis B virus (HBV) carriers and healthy controls were measured by ELISA. The levels of T lymphocyte subsets in peripheral blood were detected by flow cytometry And CD4 + CD25 + regulatory T cell percentage. Results The levels of IL-7 in CHB group, HBV-carrying group and healthy control group were (1.68 ± 0.58) ng / mL, (3.83 ± 0.62) ng / mL and (6.25 ± 0.32) ng / (31.3 ± 3.3)%, (38.1 ± 6.8)%, (43.2 ± 9.4)% respectively in the CHB group, HBV-carrying group and healthy control group ), CD8 + T lymphocytes were (24.8 ± 5.2)%, (28.1 ± 3.4)% and (30.5 ± 8.6)% respectively; CD4 + CD25 + regulatory T cells were (10.9 ± 1.3)% and 1.0)%, (4.2 ± 1.1)%. The level of IL-7 in CHB group was significantly lower than that in HBV-carrying group and healthy control group (P <0.05), and was positively correlated with the percentage of CD8 + T cells (r2 = 0.63, P <0.05) and negatively correlated with the percentage of CD4 + CD25 + regulatory T cells (r3 = -0.43, P <0.05). Conclusions IL-7 in peripheral blood of CHB patients negatively regulates regulatory T cells and promotes cellular immunity of CD8 + T cells.