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目的 :探讨人羊水在体外刺激自体血细胞释放前列环素 (PGI2 )、血栓素A2 (TXA2 )和白三烯C4(LTC4 )等花生四烯酸代谢物的作用。方法 :取产妇羊水与自体血进行培养 ,用放射免疫分析法检测血中血栓素B2 (TXB2 )和 6 -酮前列腺素F1α(6 -Keto -PGF1α)的含量 ,用酶联免疫法检测LTC4 。结果 :羊水能刺激血细胞释放TXA2 和LTC4 ,胎粪污染的羊水作用更为明显。TXB2 的含量由加羊水培养前的 (6 3.5± 5 2 .0 )ng/L增加到培养后的 (189.1± 10 2 .0 )ng/L(P <0 0 1) ,用胎粪污染羊水与血培养后增加到 (2 89.2± 113.2 )ng/L(P <0 0 1) ;LTC4 的含量由培养前的 (4 0 .1± 39.7)ng/L增加到培养后的 (2 93.5± 2 0 6 .1)ng/L(P <0 0 1) ,胎粪污染组增加到(387.2± 2 14.6 )ng/L(P <0 0 1) ,但前列环素仅有轻度增加 ,无显著性差异 (P >0 0 5 )。结论 :羊水能刺激血细胞释放花生四烯酸类生物活性物质 ,使其正常的平衡状态被破坏 ,可能与羊水栓塞的发生机理有关。
AIM: To investigate the effect of human amniotic fluid on the release of arachidonic acid metabolites such as prostacyclin (PGI2), TXA2 and LTC4 from autologous blood cells in vitro. Methods: Maternal amniotic fluid and autologous blood were used for the culture. The levels of TXB2 and 6 - keto - PGF1α in the blood were measured by radioimmunoassay. The expression of LTC4 was detected by enzyme - linked immunosorbent assay. Results: Amniotic fluid stimulated the release of TXA2 and LTC4 from blood cells. The amniotic fluid contaminated by meconium was more obvious. The level of TXB2 increased from (6.53 ± 5.2) ng / L to (189.1 ± 102.0) ng / L (P <0.01) before culture with amniotic fluid, (2 89.2 ± 113.2) ng / L (P 0 01), and the level of LTC4 increased from (41 0 ± 39.7) ng / L before culturing to (23 93.5 ± (P <0.01), and increased to (387.2 ± 2 14.6) ng / L (P 0 01) in meconium-stained group with only slight increase of prostacyclin (P <0.01) No significant difference (P> 0.05). CONCLUSION: Amniotic fluid can stimulate the release of arachidonic acid bioactive substances by blood cells, which can destroy the normal equilibrium state and may be related to the pathogenesis of amniotic fluid embolism.