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目的观察14-3-3zeta和p-Bad在T1期非小细胞肺癌(NSCLC)中的表达,探讨两者在非小细胞肺癌发展中的作用及相互关系。方法取110例非小细胞肺癌及癌旁正常肺组织存档蜡块和50例新鲜NSCLC及癌旁正常肺组织标本,采用免疫组织化学和免疫印迹法(Western blotting)检测14-3-3zeta和p-Bad蛋白的表达。结果与正常肺组织相比,14-3-3zeta在NSCLC中的表达显著增强,与肺癌的分化程度和淋巴结转移有关,而与患者的年龄、性别及病理学分型无关。p-Bad在正常肺组织中轻微表达,而在NSCLC中表达显著增强。且其表达与患者的年龄、性别及病理学分型无关,但与癌组织分化程度、淋巴结转移相关。结论 14-3-3zeta蛋白与p-Bad与T1期NSCLC发展、侵袭和转移密切相关。
Objective To observe the expression of 14-3-3zeta and p-Bad in T1-stage non-small cell lung cancer (NSCLC) and to explore their roles in the development of non-small cell lung cancer (NSCLC). Methods Totally 110 non-small cell lung cancer and adjacent normal lung tissues were preserved in paraffin blocks and 50 fresh NSCLC and adjacent normal lung tissues. Immunohistochemistry and Western blotting were used to detect 14-3-3zeta and p -Bad protein expression. Results Compared with normal lung tissue, the expression of 14-3-3zeta in NSCLC was significantly increased, which was correlated with the differentiation of lung cancer and lymph node metastasis, but not with age, gender and pathological type. p-Bad is slightly expressed in normal lung tissue, whereas it is significantly increased in NSCLC. The expression was not related to the age, sex and pathological type of the patients, but it was related to the degree of differentiation and lymph node metastasis. Conclusion The 14-3-3zeta protein is closely related to the development, invasion and metastasis of p-Bad and T1-stage NSCLC.