补体C5b-9复合物诱导大鼠肾系膜细胞iNOS mRNA表达的实验研究

来源 :中国免疫学杂志 | 被引量 : 0次 | 上传用户:wpqh918
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目的 观察人血清补体C5b-9复合物对大鼠肾小球系膜细胞(MC)表达诱生性一氧化氮合酶(iNOS)mRNA的影响。方法 首先提取人血清补体C5b-9复合物,然后用人C5b-9复合物刺激培养的大鼠MC,检测MC在受C5b-9复合物刺激后3、6、24和48h时iNOS mRNA的表达情况。同时检测其培养上清液中一氧化氮(NO)代谢产物-硝酸根(NO3^-)和亚硝酸根(NO2^-)含量的变化。结果 用人
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