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目的研究血卟啉衍生物(hematoporphyrin derivative,HpD)介导的光动力疗法(photodynamic therapy,PDT)对在体和离体人肺腺癌细胞A549的杀伤作用。方法以MTT法检测HpD-PDT对A549细胞增殖抑制的量效关系,应用流式细胞术分析其对细胞凋亡的影响。进一步构建A549细胞裸鼠移植瘤动物模型,通过绘制肿瘤生长曲线观察HpD-PDT对肺癌生长的抑制作用,并应用TUNEL法检测肿瘤细胞凋亡。结果HpD-PDT作用后,A549细胞增殖受抑,凋亡增加,显著高于对照组(P<0.05),且其抑制效率依赖于HpD浓度及激光剂量;裸鼠移植瘤动物模型肿瘤生长受抑、凋亡增加,也与对照组存在显著性差异(P<0.01)。结论HpD-PDT可有效杀伤肺腺癌细胞,其作用机制可能与抑制肿瘤细胞增殖、诱导细胞凋亡有关。
Objective To study the killing effect of hematoporphyrin derivative (HpD) -mediated photodynamic therapy (PDT) on human lung adenocarcinoma A549 cells in vitro and in vivo. Methods MTT assay was used to detect the dose-response relationship of HpD-PDT on A549 cell proliferation. The effect of HpD-PDT on apoptosis was analyzed by flow cytometry. To further establish the animal model of A549 cell xenograft in nude mice, observe the inhibitory effect of HpD-PDT on the growth of lung cancer by drawing the tumor growth curve, and detect the apoptosis of tumor cells by TUNEL method. Results After HpD-PDT treatment, the proliferation of A549 cells was inhibited and the apoptosis increased, which was significantly higher than that of the control group (P <0.05). The inhibitory efficiency of HpD-PDT was dependent on the HpD concentration and laser dose. , Apoptosis increased, but also with the control group there was significant difference (P <0.01). Conclusion HpD-PDT can effectively kill lung adenocarcinoma cells and its mechanism may be related to the inhibition of tumor cell proliferation and induction of apoptosis.