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目的:了解2007年-2010年萧山区分离的宋内志贺菌的病原分子生物学特征,并分析其流行规律。方法:对130株宋内志贺菌进行生化反应和血清分型鉴定,利用PCR方法检测侵袭性质粒抗原H(ipaH)基因。使用脉冲场凝胶电泳(PFGE)进行同源性分型分析,并使用BioNumerics进行聚类分析以确定菌株间的亲缘关系。结果:130株菌株均为宋内志贺菌,均具有ipaH基因。PFGE分型将菌株分成90个型别,相似性72.4%～97.9%,主要分为A、B、C三个克隆群。结论:PFGE分型结果表明2007年-2010年萧山区的宋内志贺菌来源具有多样性,A、B、C三个克隆群的菌株占据主导地位,应引起重视。
Objective: To understand the pathogenic molecular biological characteristics of Shigella sonnensis isolates from Xiaoshan in 2007-2010, and to analyze its epidemiology. Methods: 130 strains of Shigella sonnei were subjected to biochemical reaction and serotyping. The ipaH gene of invasive plasmid was detected by PCR. Homology typing was performed using pulsed-field gel electrophoresis (PFGE) and cluster analysis was performed using BioNumerics to determine the genetic relationship among strains. Results: All 130 strains were Shigella sonnei, all of which had ipaH gene. PFGE typing divided the strains into 90 types with similarities ranging from 72.4% to 97.9%. There were three clonal groups, A, B and C. Conclusion: The results of PFGE typing showed that the source of Shigella sonnei from Xiaoshan district in 2007 - 2010 was diversified. The strains of A, B and C clones dominated and should be taken seriously.