Meishan Section D in Changxing County, Zhejiang Province, China has been selected as the global stratotype of the Permian-Triassic boundary and various studies have been done at the boundary, but the gas chromatographic-mass spectrographic analysis of alkane biomarkers has not been investigated. This paper presents the results of a study of the biomarkers analyzed in a series of samples across the Permian-Triassic boundary at both Meishan Section A and Section D. The results show that the overall concentration of alkane biomarkers in the Permian-Triassic boundary strata is high in Bed 26 while it is low in Bed 27. A variety of biomarker parameters demonstrate that the main sources of organic matter in the sediment are algae and bacteria and that the depositional environment varied from weakly oxidizing to reducing during the studied interval.