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目的利用~(99)锝~m 标记的突触结合蛋白 I-C2A 片段(~(99)Tc~m-Syt I-C2A)作为放射性核素显像剂检测体内易损动脉粥样硬化斑块。方法用基因工程制备突触结合蛋白 I-C2A 片段,2-亚氨基噻吩盐酸盐(2-IT)法进行放射性核素标记,制成~(99)Tc~m-Syt I-C2A。5只新西兰白兔经胆固醇饲料喂养3个月及腹主动脉内皮损伤形成易损动脉粥样硬化斑块模型,另3只作为正常对照组。静脉注射~(99)Tc~m-Syt I-C2A 后进行活体显像,并取出腹主动脉进行体外血管显像和病理检查。结果注射显像剂后2 h,实验组腹主动脉部位放射性摄取明显增高,显影清晰,靶器官/本底(T/B)放射性比值为3.25±0.51。体外显像,腹主动脉亦可见高放射性摄取,与胸主动脉的放射性比值为8.39±1.74。实验组腹主动脉单位质量放射性摄取是正常对照组的12.6倍,是胸主动脉的10.2倍。结论 ~(99)Tc~m-Syt I-C2A 能够与易损粥样硬化斑块特异结合,并进行体外成像,有望成为无创性检测动脉易损粥样硬化斑块的有效方法。
Objective To detect fragile atherosclerotic plaque in vivo using ~ (99) technetium-m labeled synaptotagmin I-C2A fragment (~ (99) Tc ~ m-Syt I-C2A) as radionuclide imaging agent . Methods The synuclein I-C2A fragment was prepared by gene engineering and radioactive nuclide was labeled with 2-iminothiophene hydrochloride (2-IT) to make ~ (99) Tc ~ m-Syt I-C2A. Five New Zealand white rabbits were fed with cholesterol diet for 3 months and abdominal aorta endothelium injury formed vulnerable atherosclerotic plaque model, the other three were used as normal control group. Intravenous injection of ~ (99) Tc ~ m-Syt I-C2A in vivo imaging and removal of the abdominal aorta for in vitro vascular imaging and pathological examination. Results The radioactivity uptake of the abdominal aorta in the experimental group was significantly increased at 2 h after injection of the imaging agent, and the imaging developed clearly. The radioactivity ratio of target organ / background (T / B) was 3.25 ± 0.51. In vitro imaging, abdominal aorta also showed high radioactive uptake, radioactivity ratio with the thoracic aorta was 8.39 ± 1.74. The experimental group of abdominal aorta quality radioactive uptake is 12.6 times the normal control group is 10.2 times the thoracic aorta. Conclusion 99Tc-m-Syt I-C2A can specifically bind to vulnerable atherosclerotic plaques and perform in vitro imaging, which is expected to be an effective method for the noninvasive detection of vulnerable atherosclerotic plaques.