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【目的】细菌对抗生素的耐药性已成为全球公共卫生问题关注的热点。有研究表明外源添加化学物质可以增强耐药细菌对抗生素的敏感性。本研究比较了3种化学物质葡萄糖、丙氨酸、甘油对增强副溶血性弧菌抗生素敏感性的作用效果。【方法】在亚抑菌浓度抗生素胁迫条件下,通过比较副溶血性弧菌在添加终浓度为10 mmol/L葡萄糖、丙氨酸、甘油后细菌存活率随时间的变化水平,来观察弧菌对亚抑菌浓度抗生素敏感性作用效果的改变,并采用氧化磷酸化解偶联剂CCCP对实验结果进行验证。【结果】发现3种外源化学物质均能增强亚抑菌浓度氨基糖苷类抗生素对副溶血性弧菌的杀菌能力,其中外源添加葡萄糖对增强亚抑菌浓度卡那霉素的杀菌能力最为显著,而对其他种类抗生素的杀菌能力则无明显增强作用。加入氧化磷酸化解偶联剂CCCP后可消除由外源化学物质引发的弧菌抗生素敏感性作用增强的现象。【结论】通过调节细菌细胞代谢水平可提高耐药副溶血性弧菌对氨基糖苷类抗生素的敏感性,对多重耐药副溶血性弧菌的防控具有一定的实际应用价值。
【OBJECTIVE】 Bacterial resistance to antibiotics has become a hot issue in global public health issues. Studies have shown that adding exogenous chemicals can enhance the sensitivity of antibiotic-resistant bacteria to antibiotics. This study compared the effects of three chemicals, glucose, alanine and glycerol, on the enhancement of antibiotic susceptibility to Vibrio parahaemolyticus. 【Method】 Vibrio parahaemolyticus was isolated from Vibrio parahaemolyticus under the conditions of sub-bacteriostatic concentration of antibiotic stress compared with the change of the viability of the bacterium after the addition of 10 mmol / L glucose, alanine and glycerol. The effect of antibiotic sensitivity to sub-bacteriostatic concentration was changed, and the experimental results were verified by the oxidative phosphorylation uncoupler CCCP. 【Result】 Three kinds of exogenous chemicals were found to enhance the bactericidal activity of sub-bacteriostatic concentrations of aminoglycoside antibiotics on Vibrio parahaemolyticus. Among them, exogenous glucose was the most effective bactericide to enhance the concentration of kanamycin Significant, while the other types of antibiotics bactericidal capacity was no significant enhancement. The addition of oxidative phosphorylation decoupling agent CCCP eliminates the effect of increased susceptibility to vibrios caused by foreign chemicals. 【Conclusion】 The sensitivity of Vibrio parahaemolyticus to aminoglycoside antibiotics can be increased by regulating bacterial cell metabolism, which is of practical value for the prevention and control of multi-drug resistant Vibrio parahaemolyticus.