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目的和方法 为研究细胞因子在实验性变态反应性脑脊髓炎 (EAE)及多发性硬化 (MS)发病机制中的作用 ,用 CTL L细胞株滴定法检测了 EAE动物脾细胞培养上清中白细胞介素 - 2 (IL- 2 )的生物学活性 ,用EL ISA分别检测了白细胞介素 - 4 (IL - 4 )及干扰素 -γ(IFN-γ)水平。结果 EAE组 IL - 2活性及 IFN-γ水平均显著高于正常对照组 (均为 P<0 .0 5) ,而其 IL- 4水平显著低于对照组 (P<0 .0 1)。结论 IL- 2、IFN- γ等细胞因子参与了 EAE的发病 ,并对 IL- 4的产生有抑制作用。
Purpose and Methods To investigate the role of cytokines in the pathogenesis of experimental allergic encephalomyelitis (EAE) and multiple sclerosis (MS), CTL L cell titration was used to detect the leukocyte The levels of IL - 4 and IFN - γ were measured by ELISA. Results The levels of IL - 2 and IFN - γ in EAE group were significantly higher than those in normal control group (all P <0.05), while the levels of IL - 4 in EAE group were significantly lower than those in control group (P <0.01). Conclusion Cytokines such as IL-2 and IFN-γ are involved in the pathogenesis of EAE and inhibit IL-4 production.