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目的 在原核细胞中表达融合蛋白 h TNFα-h Pgn K5 并鉴定其活性 .方法 构建 h TNFα-h Pgn K5 基因的表达载体 ,并在大肠杆菌 DH5α中进行表达 .以 MTT法测定该融合蛋白体外抑制肿瘤细胞或血管内皮细胞增殖的活性 .结果 成功地构建了h TNFα-h Pgn K5 表达载体并获得表达 .
Objective To express fusion protein h TNFα-h Pgn K5 and identify its activity in prokaryotic cells. METHODS: The expression vector of h TNFα-h Pgn K5 gene was constructed and expressed in E.coli DH5α. The in vitro inhibition of the fusion protein was determined by MTT assay. The activity of proliferation of tumor cells or vascular endothelial cells. Results The h TNFα-h Pgn K5 expression vector was successfully constructed and expressed.