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目的：了解人ＴＭＪ滑膜细胞正常状态下参与细胞因子合成能力及ＩＬ－１对这方面的影响和调控作用。方法：采用体外培养人ＴＭＪ滑膜细胞（ＳＭＣ）及４种细胞因子和抗ＩＬ－２ｒ抗体，以ＡＢＣ法，对正常状态下ＳＭＣ的培养细胞和经ＩＬ－１作用４ｄ后的细胞进行免疫组织化学染色，观察细胞浆、膜上阳性物质分布情况。结果：正常状态ＳＭＣ对ＩＬ－１、ＩＬ－２ｒ（白介素２受体）、ＩＬ－６、ＴＮＦ表达均呈阳性或弱阳性，主要分布在胞浆内，但经ＩＬ－１作用后，几种抗体染色的阳性细胞比例大幅增加，阳性颗粒密度也增加，整个视野内增加一倍以上。结论：正常状态下，ＳＭＣ就有少量细胞参加ＩＬ－１、ＩＬ－６、ＴＮＦ的合成，ＩＬ－１对这种合成能力有正相调节作用
Objective: To understand the human TMJ synovial cells under normal conditions involved in cytokine synthesis and IL-1 in this regard and regulatory role. Methods: Cultured human TMJ synoviocytes (SMCs) and 4 kinds of cytokines and anti-IL-2r antibodies in vitro were used to detect the expression of SMC in cultured cells and cells after 4 days of IL-1 treatment Chemical staining, observation of cytoplasm, membrane positive substance distribution. Results: The expression of IL-1, IL-2R (IL-2R), IL-6 and TNF were all positive or weakly positive in normal SMC, mainly distributed in the cytoplasm. However, The proportion of positive cells stained with antibodies increased significantly, and the positive particle density also increased, more than double the entire field of view. CONCLUSIONS: SMCs have a small number of cells participating in the synthesis of IL-1, IL-6 and TNF under normal conditions. IL-1 has a positive effect on this synthesis ability