目的:探讨枸杞多糖(Lycium barbamm polysaccharide,LBP)对紫外线(UV)辐射后HaCaT细胞内活性氧清除的作用及其机制,为UV防护剂的研发提供理论依据。方法:根据不同实验具体要求创建不同程度UVA(长波紫外线)和UVB(中波紫外线)损伤HaCaT细胞模型,实验分为空白组、UV辐射组、UV+LBP干预组、LBP组,采用MTT法检测不用浓度枸杞多糖对HaCaT细胞的增殖活性,确定适宜无毒性的LBP浓度;通过酶标仪法测定LDH漏出量;利 Objective: To investigate the effect of lycium barbam polysaccharide (LBP) on reactive oxygen species scavenging in HaCaT cells after ultraviolet (UV) radiation and to provide a theoretical basis for the development of UV protective agents. Methods: HaCaT cell models were established according to the different requirements of different experiments. The cells were divided into blank group, UV radiation group, UV + LBP intervention group and LBP group. MTT assay Without the concentration of LBP on HaCaT cell proliferation activity to determine appropriate non-toxic concentration of LBP; LDH leakage by microplate reader;