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以褐鳟(Salmo trutta)日本品系和亚东鲑群体为研究对象,通过测定上述两个群体8个微卫星标记和线粒体D-loop基因多态性,综合分析日本品系和亚东鲑群体遗传多样性及其种质来源。结果表明,褐鳟日本品系等位基因数为2~10个,亚东鲑群体为4~9个,平均有效等位基因数分别为3.1304和3.8988;观测杂合度范围分别为0.3404~0.7128和0.1020~0.8776,其中褐鳟日本品系平均观测杂合度为0.5612,亚东鲑群体为0.5510,期望杂合度分别为0.4671~0.7794和0.5216~0.7932;褐鳟日本品系多态信息含量(PIC)为0.3680~0.7440,平均为0.5964,而亚东群体PIC为0.4660~0.8370,平均为0.5926。研究分析褐鳟、亚东鲑线粒体D-loop基因序列和欧洲褐鳟已知五个地理居群D-loop基因序列,日本品系褐鳟和亚东鲑与欧洲大西洋居群褐鳟聚在一支,亲缘关系最近。
Taking the Japanese strain of Salmo trutta and the population of Asian salmon trotta as the research object, 8 microsatellite markers and mitochondrial D-loop gene polymorphisms of the above two populations were determined to analyze the genetic diversity Sexual and germplasm sources. The results showed that the number of alleles of Japanese brown trout was 2 ~ 10 and that of Asian salmon was 4 ~ 9. The average number of effective alleles was 3.1304 and 3.8988, respectively. The observed heterozygosity ranged from 0.3404 to 0.7128 and 0.1020 ~ 0.8776, respectively. The average observed heterozygosity of Japanese brown trout was 0.5612, that of Asian salmon trout was 0.5510, and the expected heterozygosities were 0.4671 ~ 0.7794 and 0.5216 ~ 0.7932 respectively. The polymorphism information content (PIC) of Japanese brown trout was 0.3680 ~ 0.7440 , With an average of 0.5964, while the Asian East PIC of 0.4660 ~ 0.8370, with an average of 0.5926. To study the mitochondrial D-loop gene sequence of brown trout and Asian brown trout and the sequence of D-loop gene of five geographic populations known to European brown trout, the Japanese strain brown trout and Asian tamarense clustered together with the European Atlantic brown trout , Kinship recently.