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神经干细胞(neural stem cells,NSCs)的定向迁移对神经系统发育和损伤后修复至关重要,但NSCs的定向迁移与NSCs的分化之间的关系鲜有研究。该研究以此为切入点,以肝细胞生长因子(hepatocyte growth factor,HGF)为趋化因子,神经干细胞系C17.2为研究对象,首先,建立了不同分化阶段的NSCs(分别分化0,12,24,72 h)的分化模型;其次,运用Boyden chamber和Dunn chamber研究了不同分化状态下的NSCs对HGF的趋化性迁移。Boyden chamber结果显示:下室加入HGF后,分化12,24 h的NSCs迁移至膜下方的细胞数目显著高于分化0,72 h的NSCs;Dunn chamber结果显示:分化12,24 h的NSCs迁移效率显著高于分化0,72 h的NSCs。这些结果表明,NSCs的分化影响其对HGF的趋化性迁移,为在临床上更有效地利用NSCs治疗各种神经系统退行性疾病提供了理论依据。
Directional migration of neural stem cells (NSCs) is crucial for neurological development and post-injury repair, but there is little research into the relationship between the directional migration of NSCs and the differentiation of NSCs. Based on this study, hepatocyte growth factor (HGF) as a chemokine and neural stem cell line C17.2 were selected as research objects. Firstly, NSCs with different differentiation stages were established (differentiated 0,12 , 24, 72 h). Secondly, Boyden chamber and Dunn chamber were used to study the chemotactic migration of HGF by NSCs under different differentiation conditions. The results of Boyden chamber showed that the number of NSCs differentiated to 12 and 24 h after migration into subretinal cells was significantly higher than that of NSCs differentiated to 0 and 72 h after adding HGF in the lower chamber. The results of Dunn chamber showed that the migration efficiency of NSCs at 12 and 24 h Significantly higher than that of NSCs differentiated for 0 and 72 h. These results indicate that the differentiation of NSCs affects the chemotactic migration of HGF and provides a theoretical basis for the more effective clinical treatment of various types of neurodegenerative diseases using NSCs.