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目的分析血根碱(SAN)对钉螺肝脏细胞凋亡的影响,并探讨其杀螺作用机理。方法配制6 mg/L SAN,25℃浸泡钉螺48 h,设清水对照组,解剖各组活钉螺,分离肝脏;将钉螺肝脏匀浆,测定肝脏过氧化物酶(POD)、总超氧化物歧化酶(T-SOD)活性和谷胱甘肽(GSH)含量;分离钉螺细胞,流式细胞术检测钉螺肝脏细胞凋亡;采用独立样本t检验对测定结果进行统计学分析。结果 SAN组钉螺肝脏的POD、T-SOD活性分别为(12.750±0.889)U/mgprot、(6.200±1.043)U/mgprot;清水对照组分别为(4.903±0.086)U/mgprot、(0.776±0.553)U/mgprot,两组间POD、T-SOD活性的差异均有统计学意义(P均<0.05)。SAN组钉螺肝脏GSH含量为(2.512±0.022)mg/gprot,清水对照组为(0.588±0.086)mg/gprot,差异有统计学意义(P<0.05);SAN组和清水对照组钉螺肝脏细胞的早期凋亡率分别为(4.633%±0.058%)和(3.500%±0.173%),晚期凋亡率分别为(3.400%±0.100%)和(1.400%±0.200%),差异均有统计学意义(P均<0.05)。结论 SAN使钉螺肝脏POD、T-SOD活性和GSH含量升高,诱导钉螺肝脏细胞凋亡。
Objective To analyze the effect of SAN on apoptosis of hepatocytes in the snail and discuss the mechanism of its killing effect. METHODS: Six mg / L SAN was prepared and soaked in snails at 25 ℃ for 48 h. The control group was divided into two groups: the control group, the live snail and the liver were dissected. The liver of the snail was homogenized and the activities of peroxidase (POD), total superoxide dismutase (T-SOD) and glutathione (GSH) were detected. The snail cells were isolated and the apoptotic cells were detected by flow cytometry. The results of the independent samples t test were used for statistical analysis. Results The activities of POD and T-SOD in the liver of snail were (12.750 ± 0.889) U / mgprot and (6.200 ± 1.043) U / mgprot respectively in the control group and 4.903 ± 0.086 U / mgprot in the water control group (0.776 ± 0.553 ) U / mgprot. The differences of POD and T-SOD activity between the two groups were statistically significant (all P <0.05). The content of GSH in the liver of SAN group and control group were (2.512 ± 0.022) mg / gprot and (0.588 ± 0.086) mg / gprot in the control group, respectively, with statistical significance The early apoptotic rates were (4.633% ± 0.058%) and (3.500% ± 0.173%) respectively, and the late apoptotic rates were (3.400% ± 0.100%) and (1.400% ± 0.200%, respectively) (P <0.05). Conclusions SAN can increase the activity of POD, T-SOD and the content of GSH in the liver of the snail and induce the apoptosis of hepatocytes in the snail.