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应用差异聚合酶链反应方法，检测９０例胃癌（早期胃癌３０例）组织中ｃ－ｅｒｂＢ－２基因扩增情况，以探索该基因扩增与胃癌预后关系及临床应用价值。结果表明，２２．２％（２０／９０）胃癌组织中存在ｃ－ｅｒｂＢ－２扩增，其与淋巴结转移关系密切（Ｐ＜０．０５）；而与肿瘤组织学类型及浸润深度无关。在胃癌原发灶及对应受侵淋巴结中，两者ｃ－ｅｒｂＢ－２扩增无明显差异。生存期分析显示，ｃ－ｅｒｂＢ２扩增者预后差，５年生存期短（Ｐ＜０．０５），其中在３０例早期胃癌中，有４例ｃ－ｅｒｂＢ－２扩增，５年内死亡２例，全部为扩增者．本文结果提示ｃ－ｅｒｂＢ－２检测可能有助于胃癌预后判断及在早期胃癌中进行高危组别筛选。
Using differential polymerase chain reaction method, we detected the amplification of c-erbB-2 gene in 90 cases of gastric cancer (early gastric cancer 30 cases) to explore the relationship between the gene amplification and the prognosis of gastric cancer and its clinical application value. The results showed that there was c-erbB-2 amplification in 22.2% (20/90) of gastric cancer tissues, which was closely related to lymph node metastasis (P<0.05), but not related to histological type and depth of invasion. There was no significant difference in the amplification of c-erbB-2 between primary gastric cancer and corresponding invaded lymph nodes. The survival analysis showed that the prognosis of c-erbB2 amplification was poor, and the 5-year survival period was short (P<0.05). Among 30 cases of early gastric cancer, there were 4 cases of c-erbB-2 amplification and 5 years of death 2 Cases, all amplification. The results of this study suggest that c-erbB-2 may be helpful for the prognosis of gastric cancer and screening for high-risk groups in early gastric cancer.