论文部分内容阅读
目的常用大鼠移植性肝癌模型制作方法存在诸多不足,通过对直接注射法的改良以解决其缺陷。方法将90只Wistar大鼠随机分成3组,每组30只,分别以直接注射法、瘤块种植法和改良注射法进行模型制作,观察动物模型的肿瘤生长情况和动物模型的生存情况。结果术后第8天对3组大鼠进行比较,改良注射法模型组(C组)的肿瘤最大直径、体积和质量与直接注射法模型组(A组)、瘤块种植法模型组(B组)间差异并不显著(P>0.05),具有可行性,在肿瘤移植的成功率方面以改良注射法模型组最高;在腹水和腹壁瘤发生率方面C组明显低于A、B两组(P<0.01);生存时间长于A、B两组(P<0.01)。结论基于易行性和重复性考虑而产生的改良注射法,优化了直接注射法的实验操作方法、弥补了直接注射法的缺陷,很好地控制了实验条件,既拥有直接注射法的易行性,又提升了其可重复性,同时保证了动物模型的适用性和可靠性。
OBJECTIVE: There are many deficiencies in the method of making the model of transplantation hepatocarcinoma in rats. The defect of the method is solved by the direct injection method. Methods Totally 90 Wistar rats were randomly divided into 3 groups with 30 rats in each group. The rats were directly injected into the tumor and implanted into the tumor. The animals were sacrificed and the animals were sacrificed. Results The maximum diameter, volume and mass of the model group (group A), model group (B), model group Group) was not significant (P> 0.05), it is feasible in the success rate of tumor transplantation improved injection model group was the highest; in the incidence of ascites and abdominal aneurysm C group was significantly lower than the A and B groups (P <0.01). The survival time was longer than that in A and B groups (P <0.01). Conclusion Based on the feasibility and repeatability of the modified injection method, the direct injection method was optimized to make up for the shortcomings of the direct injection method and to control the experimental conditions well. In addition to the direct injection method, Sexual, but also enhance its repeatability, while ensuring the applicability and reliability of the animal model.