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目的 观察FTY72 0对近交系小鼠外周血淋巴细胞的影响。方法 选用近交系雄性C5 7BL/ 6小鼠 ,连续口服FTY72 0 (3mg·kg-1·d-1) 14d。每只动物于服药后 2、4、6、8、12、2 4h以及停药后 1、2、3、4、5、6周自尾静脉采血 ,计数淋巴细胞数。结果 服用FTY72 0后 ,小鼠外周血淋巴细胞迅速减少 ,2h后即由服药前的 (870 0± 6 5 6 ) / μl降至 (3783± 176 ) / μl(P <0 .0 1) ,并于 4h降至最低值 (2 4 6 7± 2 5 2 ) / μl,之后基本保持稳定。停用FTY72 0后小鼠外周血淋巴细胞没有立刻开始恢复 ,在低水平徘徊 2周后 ,第 3周淋巴细胞数开始出现明显回升 ,停药后第 6周恢复到 (7833± 76 4 ) / μl,与服药前相比 ,差异无显著性 (P >0 .0 5 )。结论 FTY72 0起效迅速 ,特异性强 ,且作用可逆。
Objective To observe the effect of FTY72 0 on peripheral blood lymphocytes in mice of inbred line. Methods Inbred male C5 7BL / 6 mice were selected and treated with FTY72 0 (3 mg · kg-1 · d-1) for 14 days. Each animal was taken from the tail vein at 2, 4, 6, 8, 12, 24 h and 1, 2, 3, 4, 5, and 6 weeks after the drug withdrawal, and the number of lymphocytes was counted. Results After FTY72 0 administration, the number of peripheral blood lymphocytes in mice rapidly decreased from (870 ± 6 56) / μl to (3783 ± 176) / μl 2 h before administration (P <0.01) And dropped to the lowest value (2467 ± 2552) / μl at 4h, after which it remained basically stable. After FTY72 0 was stopped, the peripheral blood lymphocytes of mice did not start to recover immediately. After 2 weeks of low level, the number of lymphocytes started to rise significantly at the 3rd week and returned to 7833 ± 76 4 / μl, there was no significant difference between before and after treatment (P> 0.05). Conclusion FTY72 0 has rapid onset, specificity and reversible effects.