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目的探讨中枢内源性乙酰胆碱(ACh)对颈动脉窦压力感受器反射(CSR)的作用。方法 SD大鼠麻醉后孤离其双侧颈动脉窦区,将不同窦内压(ISP)与其对应的平均动脉压(MAP)值进行Logistic曲线方程拟合,求得ISP-MAP关系曲线及其反射特征参数,观察侧脑室注射不同剂量的拟胆碱药毒扁豆碱(PHY)对CSR的影响。结果侧脑室分别注射低(1.8 mmol/L)、中(3.6 mmol/L)、高(5.4 mmol/L)剂量的PHY可导致ISP-MAP关系曲线在ISP 120～280 mmHg区间明显依次上移(P<0.05),ISP-增益关系曲线中部明显依次下移(P<0.05),反射参数中调定点、阈压、饱和压和最大增益时的窦内压值明显增大(P<0.05),MAP反射变动范围及反射最大增益明显减小(P<0.05)。结论 PHY引起中枢内源性ACh增多,对CSR具有抑制性重调定作用,且这种效应呈现明显的剂量依赖性。
Objective To investigate the effect of central endogenous acetylcholine (ACh) on carotid sinus baroreceptor reflex (CSR). Methods Sprague-Dawley rats were isolated from bilateral carotid sinus and anesthetized. Logistic curve fitting was performed between different sinus pressure (ISP) and corresponding mean arterial pressure (MAP), and the relationship curve between ISP-MAP and its Reflecting the characteristic parameters, we observed the effect of intracerebroventricular injection of different doses of cholinergic physostigmine (PHY) on CSR. Results The intracerebroventricular injection of low (1.8 mmol / L), medium (3.6 mmol / L) and high (5.4 mmol / L) PHYs resulted in significant up-shift of ISP- (P <0.05), and the central part of ISP-gain curve shifted downwards in turn (P <0.05). The sinusoidal pressure value at the set point, threshold pressure, saturation pressure and maximum gain in the reflex parameters increased significantly The range of MAP reflection and the maximum gain of reflection were significantly reduced (P <0.05). Conclusion PHY induced central endogenous ACh increased, inhibitory re-tuning of CSR, and this effect showed a significant dose-dependent.