论文部分内容阅读
5-羟色胺是颅内最强的血管收缩剂之一,对维持脑血管结构的完整性有着十分重要的意义,在脑血管病研究时,常需了解5-羟色胺递质的变化与血管形态间的关系.文献报道了应用双PAP及ABC法在同一切片上显示5-羟色胺与脑血管的方法,为研究脑血管病的发生,发展和研究5-羟色胺之间的关系提供了一个真实、直观的研究手段,但由于存在着切片厚,抗体穿透速度有限,实验操作时间长及背景染色深等缺点,本文根据微波辐射能加速组织固定,染色和降低背景染色等优点我们对原方法进行了改进,取得了满意结果,现介绍如下.
Serotonin is one of the strongest intracranial vasoconstrictors and is of great importance in maintaining the integrity of cerebrovascular structures. In the study of cerebrovascular disease, it is often necessary to know the relationship between changes in serotonin transmitter and vascular morphology Reported in the literature using the double PAP and ABC method on the same slice shows serotonin and cerebrovascular method, in order to study the occurrence of cerebrovascular disease, the development and study of the relationship between serotonin provides a true and intuitive However, due to the disadvantages such as thick slice, limited penetration rate of antibody, long time of experimental operation and deep staining of the background, this article based on the advantages of microwave radiation to accelerate tissue fixation, dyeing and reduce background staining, etc. The original method Improve and have achieved satisfactory results, are presented below.