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目的 :研究雄激素受体 (AR)亚型在前列腺组织中的表达及内外带分布。方法 :采用聚丙烯酰胺凝胶等电聚焦电泳 (IEF)技术 ,检测了前列腺组织标本 2 0例。结果 :前列腺组织有 3种AR亚型 ,等电点 (PI)分别在 6 5、6 0和 5 3,3种亚型均表达 13例 ,占 6 5% ,所有标本均表达PI 6 5亚型 ;前列腺内带和外带均有 3种亚型表达 ,个体间存在差异 ;定量分析表明 ,前列腺内外带AR含量 ,外带高于内带 ,但无统计学意义 (P >0 .0 5) ,PI 6 0亚型浓度在外带明显高于内带 (P <0 .0 5)。结论 :前列腺AR存在亚型并且具有带性分布特征。
Objective: To study the expression and distribution of androgen receptor (AR) subtype in prostatic tissue. Methods: Polyacrylamide gel isoelectric focusing (IEF) technique was used to detect 20 specimens of prostate tissue. Results: There were 3 types of AR subtype in prostate tissue. The isoelectric point (PI) of 13, 6 6 and 5 5 in 3, 6, 6 and 5 subtypes, respectively, accounted for 65% and all the specimens expressed PI 65 There were three subtypes in the inner and the outer of the prostate, which were different among individuals. Quantitative analysis showed that the contents of AR in the inner and outer prostatic were higher than those in the inner zone, but there was no statistical significance (P> 0.05) ), And the concentration of PI 6 0 subtype was significantly higher in outer zone than in inner zone (P <0.05). Conclusion: There are subtypes of AR in prostate and have zonal distribution.