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目的研究甘肃岷县不同海拔对当归渗透调节物质的影响,为当归生态适宜区选择提供参考。方法采用田间试验和实验室实验相结合的方法研究不同海拔当归体内丙二醛(MDA)、脯氨酸(Pro)和可溶性糖(SS)含量变化,从细胞渗透调节方向探索当归适宜种植海拔。结果随海拔升高,MDA,Pro含量先降低后增加,而SS含量保持降低趋势,海拔间差异显著。其中MDA含量在海拔2 570 m和2 780 m分别显著低于海拔2 360 m的52.4%和38.1%,海拔2 570 m显著低于海拔2 780 m的23.1%;Pro含量在海拔2360 m和海拔2 780 m分别显著高于海拔2 570 m的12.4%和13.6%;SS含量在海拔2 360 m显著高于海拔2 780 m的17.9%,而与海拔2 570 m间差异不显著。结论海拔升高可降低当归体内MDA,Pro和SS含量,利于细胞膜稳定,故从当归细胞渗透调节角度分析,在2 300~2 800 m范围内可提升当归种植海拔,扩大当归生态区域,降低连作障碍。
Objective To study the effects of altitude on the osmolytes of Angelica sinensis in Minxian County, Gansu Province, and to provide reference for the selection of suitable areas for Angelica sinensis. Methods The contents of malondialdehyde (MDA), proline (Pro) and soluble sugar (SS) in Angelica sinensis from different altitudes were studied by a combination of field experiments and laboratory experiments to explore the suitable altitude for planting Angelica sinensis from the direction of cell osmosis. Results As the altitude increased, the content of MDA and Pro decreased at first and then increased, while the content of SS decreased while the difference of altitude was significant. Among them, MDA content was significantly lower than 52.4% and 38.1% at an altitude of 2 570 m and 2 780 m, respectively, and an elevation of 2 570 m was significantly lower than 23.1% at an altitude of 2 780 m. The content of Pro at the altitude of 2360 m and elevation 2 780 m were significantly higher than 12.4% and 13.6% respectively at an altitude of 2 570 m. The content of SS at 2 360 m above sea level was significantly higher than that of 1780% at an altitude of 2 780 m, but not significantly different from that at 2 570 m. Conclusion Elevated altitude can reduce the contents of MDA, Pro and SS in Angelica sinensis, which is conducive to the stability of the cell membrane. Therefore, from the point of view of regulating osmotic adjustment of Angelica sinensis, the elevation of Angelica sinensis can be increased in the range of 2 300 ~ 2 800 m, obstacle.