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目的：探讨川芎唪对再生障碍性贫血（再障）骨髓微环境的可能作用及其机制．方法：建立免疫介导的再障小鼠模型，胃饲川芎嗪注射液４ｍｇ／次，１天２次，第１０天用氧分压传感针测定活体尺骨骨髓氧分压（ＰｂＯ２t），再观察其骨髓切片组织学，体外成纤维细胞集落形成单位（ＣＦＵ－Ｆ），培养基质细胞层的粘附能力．结果：川芎唪治疗组尺骨中段ＰｈＯ２１０．３２±１．２７ＫＰａ较正常组（１２．３５±１．０５Ｋｐａ）低，Ｐ＜０．０１；而显著高于对照的再障组（４．３２±２．８６ＫＰａ）Ｐ＜０．００１．再障组骨髓微血甲扩张、断裂、淤血，造血组织容量百分率２４．９±９．６％．川芎嗪组微血管较清楚、完整、无断裂及淤血，造血组织容量百分率为５２．８±１５．６％．再障胡ＣＦＵ－Ｆ１２．５±７．３明显低于川芎嗪组（３１．５±１０．６），ｐ＜０．０１．川芎嗪组体外培养基质细胞层粘附正常小鼠骨髓有核细胞的能力为７２．７±７．８％，与正常组（７３．４±３．４％）无差异，明显高于再障组（５６．２±９．８％），Ｐ＜０．０１．结论：川芎嗪能促进再障小鼠骨髓微血管的修复，增加微环境供氧，促进基质细胞生长及其粘附功能．川芎嗪通过改善骨髓微环境而使骨髓造血细胞增生．
Objective: To explore the possible effect and mechanism of Chuanxiong on bone marrow microenvironment of aplastic anemia (Aplastic anemia). METHODS: An immune-mediated mouse model of aplastic anemia was established. The stomach was fed with tetramethylpyrazine for 4 mg every 2 times a day. On the 10th day, oxygen partial pressure of living ulna was measured with oxygen partial pressure sensing needle (PbO2t). 3. Observe the histology of bone marrow section, in vitro fibroblast colony forming unit (CFU-F), adhesion ability of culture medium cell layer. RESULTS: PhO210.32±1.27KPa in the middle ulna of the Chuanxiong-treated group was lower than that in the normal group (12.35±1.05Kpa), P<0.01, but significantly higher than that in the control group (4.32±2) .86KPa)P<0.001. In the aplastic anemia group, the hematopoietic expansion, rupture and congestion of bone marrow were observed. The hematopoietic tissue capacity percentage was 24.9±9.6%. Ligustrazine group microvessels clear, complete, no fracture and congestion, hematopoietic capacity was 52.8±15.6%. Atrophic hupe CFU-F12.5±7.3 was significantly lower than the Ligustrazine group (31.5±10.6), p<0.01. The ability of the ligustrazine group to adhere to bone marrow nucleated cells of normal mice in vitro was 72.7±7.8%, which was no different from the normal group (73.4±3.4%) and significantly higher than that of the aplastic anemia Group (56.2±9.8%), P<0.01. Conclusion: Tetramethylpyrazine can promote the repair of bone marrow microvessels in mice with aplastic anemia, increase microenvironmental oxygen supply, and promote the growth and adhesion of stromal cells. Ligustrazine promotes bone marrow hematopoiesis by improving the bone marrow microenvironment.