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目的:研究中药泽泻对小鼠前体脂肪细胞3T3-L1细胞形态及凋亡的影响。方法:体外培养小鼠前体脂肪细胞,显微镜观察评价细胞形态学变化,MTT法评价细胞增殖情况。AO/EB染色法评价前体脂肪细胞凋亡。结果:MTT法和凋亡检测揭示不同浓度的泽泻对前体脂肪细胞的增殖有抑制作用,呈现浓度和时间依赖性;同时促进细胞凋亡。结论:中药泽泻可能促进前体脂肪细胞凋亡。这可能为筛选具有减肥作用的细胞提供更多理论支持
Objective: To study the effect of traditional Chinese medicine Alisma orientale on the morphology and apoptosis of 3T3-L1 preadipocytes in mice. Methods: Mouse preadipocytes were cultured in vitro. Morphological changes of cells were evaluated by microscopy. Cell proliferation was evaluated by MTT assay. Apoptosis of preadipocytes was evaluated by AO / EB staining. Results: MTT assay and apoptosis assay revealed that Alzheimer’s disease with different concentrations inhibited the proliferation of preadipocytes in a concentration-and time-dependent manner and promoted apoptosis at the same time. Conclusion: Alisma orientalis may promote the apoptosis of preadipocytes. This may provide more theoretical support for screening cells with weight loss