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采用高传能线密度(LET)的12C6+离子束和低LET的X射线辐照人正常肝细胞系HL-7702细胞,利用微卫星不稳定性(MSI)检测来分析直接受照射细胞和通过转移培养基方式旁细胞传代八代子细胞以MSI表征的远后效应。实验结果表明,12C6+离子束诱导的远后效应较X射线的低;旁细胞的远后效应较直接受照射细胞的高;辐射引起的MSI与杂合性丢失(LOH)的发生率具有位点特异性。结果提示,重离子放射治疗较X射线放射治疗对正常组织引发的辐射风险要小,可通过对MSI高发位点的筛选来评估放疗后患者长期生存状况和二次癌症发生风险。
Human normal hepatocyte cell line HL-7702 was irradiated with high energy linear density (LET) 12C6 + ion beam and low LET X-ray, analyzed directly by irradiated cells by microsatellite instability (MSI) Subsequent media effects of metaphase subcultured by MSI in MSI. The experimental results show that far-after effects induced by 12C6 + ion beam are lower than those of X-rays; the far-after effects of para-cells are higher than that of irradiated cells directly; the incidence of radiation-induced MSI and loss of heterozygosity (LOH) Specificity. The results suggest that heavy ion radiotherapy is less effective than X-ray radiotherapy in initiating radiation in normal tissues. Long-term survival status and risk of secondary cancer after radiotherapy can be assessed by screening MSI-prone sites.