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普通小麦种质N9134含有野生二粒小麦AS846的抗白粉病基因,该种质对陕西省关中地区白粉病流行小种关中4号表现高抗。用高感小麦白粉病的普通小麦品种陕160和陕优225与N9134杂交,F1代对白粉病表现高抗,F2代抗病和感病植株的比例符合3∶1,表明N9134苗期白粉病抗性由1对完全显性基因控制,暂定名为PmAS846。采用66个小麦SSR引物对2个抗感分离群体共176个单株进行分析,发现引物WMS37、WMS67和WMS213的扩增产物在抗感植株DNA池间存在多态性。经分离群体验证,抗病基因PmAS846与小麦染色体5BL上的微卫星位点Xgwm67连锁,遗传距离为20.6 cM,表明PmAS846可能位于5BL上。
The common wheat germplasm N9134 contained the powdery mildew resistance gene of the wild double-grain wheat AS846, which showed high resistance to the small powdery mildew Guanzhong 4 in the Guanzhong area of Shaanxi Province. The F1 hybrids showed high resistance to powdery mildew, and the proportion of disease-resistant and susceptible plants in F2 generation accorded with 3:1, indicating that the powdery mildew of N9134 seedling stage Resistance is controlled by a pair of perfectly dominant genes, tentatively named PmAS846. Sixty-six wheat SSR primers were used to analyze a total of 176 plants in two resistant populations. It was found that the amplified products of primers WMS37, WMS67 and WMS213 were polymorphic among DNA pools of resistant plants. The segregation population showed that the resistance gene PmAS846 was linked to Xgwm67, a microsatellite locus on wheat chromosome 5BL, with a genetic distance of 20.6 cM, indicating that PmAS846 may be located on 5BL.