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构建甘薯分子连锁图谱,并对重要农艺性状进行QTL定位,进而克隆相关性状的主效基因,是甘薯育种研究的重要方向。在甘薯的农艺性状中,高胡萝卜素含量是重要育种目标之一。本研究利用低胡萝卜素含量甘薯品种漯徐薯8号(母本)和高胡萝卜素含量甘薯品种郑薯20(父本)杂交,以F1分离群体240个株系为材料,构建分子连锁图谱。父本57个连锁群,图谱的总长度4 968.62 cM,标记间平均距离10.35 cM;母本64个连锁群,图谱总长度为5 313.67 cM,标记间平均距离10.76 cM。以测定的β-胡萝卜素含量为指标,用MapQTL4.0软件检测到17个与甘薯β-胡萝卜素含量相关的QTLs,其中10个定位在父本图谱上,7个定位在母本图谱上,分别解释甘薯β-胡萝卜素含量表型变异的33.1%~62.1%。研究结果有助于甘薯胡萝卜素合成相关性状基因的克隆及分子标记辅助育种体系的建立。
It is an important direction for sweet potato breeding to construct molecular linkage map of sweet potato and to locate QTL for important agronomic traits and cloned the major gene of related traits. Among the agronomic traits of sweet potato, high carotene content is one of the important breeding goals. In this study, molecular linkage map was constructed by crossing 240 sweetpotato varieties, Luo Shusan 8 (female parent) with low carotene content and sweet potato variety Zhengshu 20 (male parent) with high carotene content. The total length of the map was 4968.62 cM with an average distance of 10.35 cM between the markers. The 64 linkage groups of the maternal parents had a total length of 5 313.67 cM and the average distance between markers was 10.76 cM. 17 QTLs related to β-carotene content of sweetpotato were detected by MapQTL4.0 software based on the measured β-carotene content, of which 10 were mapped on the male parent map, 7 were mapped on the female parent map, Respectively, explaining the phenotypic variation of β-carotene in sweet potato by 33.1% -62.1%. The results are helpful to clone the genes related to the synthesis of sweet potato carotenoids and establish the molecular marker-assisted breeding system.