以壳聚糖小球为载体,采用戊二醛交联共价固定α-淀粉酶。试验结果表明,以1 g壳聚糖小球为载体,经5m L 2%戊二醛处理后,加入24 mgα-淀粉酶,30℃,在p H 6的磷酸缓冲液中固定化2 h,制备的固定化α-淀粉酶活力达1 407.6 U/g。固定化酶最适p H向酸性方向偏移,最适反应温度不变,固定化α-淀粉酶的酸碱稳定性和热稳定性均优于游离酶。 Chitosan beads as a carrier, the use of glutaraldehyde cross-linked α-amylase immobilized. The experimental results showed that 1 g of chitosan pellets were treated with 5 ml of 2% glutaraldehyde, 24 mg of α-amylase was added, immobilized in phosphate buffer of p H 6 at 30 ° C. for 2 h, The prepared immobilized α-amylase activity reached 1 407.6 U / g. The optimum pH of the immobilized enzyme shifted to the acidic direction and the optimum reaction temperature was not changed. The stability of the immobilized α-amylase was better than that of the free enzyme.