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目的研究乳铁蛋白对辐射损伤小鼠的保护作用。方法小鼠随机分成3组,空白对照组、单纯照射组、给药组。给予6.8Gy照射剂量小鼠30d观察各组存活率实验;给予2Gy照射剂量观察各组小鼠的骨髓微核率并对有核细胞进行计数。结果单纯照射组小鼠照后死亡率较高,25d全部死亡,与给药组比较存活时间差异有统计学意义(P<0.05),一般情况也有统计学意义(P<0.05)。单纯照射组照后小鼠骨髓有核细胞数明显下降,而乳铁蛋白给药组(包括照前给药和照后给药)高于单纯照射组,比较差异有统计学意义(P<0.05)。单纯照射组照后3d小鼠骨髓的微核细胞率明显升高,而乳铁蛋白给药组低于单纯照射组,比较差异有统计学意义(P<0.05)。结论乳铁蛋白无论是照前给药还是照后给药都具有较好的抗辐射作用。
Objective To study the protective effect of lactoferrin on radiation-injured mice. Methods Mice were randomly divided into three groups: blank control group, irradiation group and administration group. The mice were sacrificed at 6.8Gy for 30 days to observe the survival rate of each group. 2Gy irradiation dose was given to observe the micronuclei of bone marrow of mice in each group and the nucleated cells were counted. Results Compared with the control group, the survival rate of the mice in the irradiation group was higher than that in the control group (P <0.05), and the death rate was higher at 25 days (P <0.05). The numbers of nucleated cells in the bone marrow of the mice in the irradiation group were significantly decreased, while those in the lactoferrin group (both before and after irradiation) were higher than those in the irradiation alone group (P <0.05 ). The number of micronucleated cells in bone marrow of mice was significantly increased after 3 days irradiation, while the lactoferrin administration group was lower than that of irradiation alone group (P <0.05). Conclusion Lactoferrin has a good anti-radiation effect both before and after irradiation.