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目的:探讨建立新生儿败血症的分子生物学诊断方法。方法:分析16S rRNA核苷酸序列,设计引物及探针,选取临床常见菌株进行PCR扩增及检测;对疑为败血症的317例新生患儿分别行PCR和血培养检测。结果:PCR检测阳性率明显高于血培养(P<0.05)。以血培养为参照,PCR检测的敏感性高,特异性好。结论:建立了新生儿败血症的分子生物学诊断方法,满足临床快速、准确的要求,具有较大的推广及应用价值。
Objective: To explore the molecular diagnosis of neonatal sepsis. Methods: The nucleotide sequences of 16S rRNA were analyzed, primers and probes were designed, and common clinical strains were selected for PCR amplification and detection. 317 newborn children with suspected sepsis were detected by PCR and blood culture respectively. Results: The positive rate of PCR detection was significantly higher than that of blood culture (P <0.05). Blood culture as a reference, PCR detection of high sensitivity and good specificity. Conclusion: The molecular biological diagnostic method of neonatal sepsis is established to meet the requirements of clinical rapid and accurate, which has a great promotion and application value.