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本文用免疫荧光细胞化学、流式荧光分析等方法研究了光敏剂H_A对培养的HeLa细胞的光敏化作用。结果表明,H_A光敏化作用使细胞形态发生变化,细胞表面微绒毛丧失,细胞增殖受到抑制,上述变化随光照剂量增加而加剧,其中对肿瘤细胞的抑制作用较正常二倍体细胞更大,细胞群体中G_1期细胞下降,S期细胞增多。H_A的光敏化作用还使胞质微管变稀疏,微管中心的亮荧光近乎消失。以上实验说明,H_A的光敏作用对培养细胞的作用引起细胞膜、细胞骨架的损伤,使细胞周期部分阻断于S期,从而抑制了细胞的增殖。
In this paper, immunofluorescence cytochemistry and flow cytometry were used to study the photosensitizing effect of photosensitizer H_A on cultured HeLa cells. The results showed that the photosensitivity of H_A changed the cell morphology, the loss of microvilli on the cell surface and the inhibition of cell proliferation. The above changes were exacerbated with the increase of light dose, and the inhibitory effect on tumor cells was more than that of normal diploid cells. Group G_1 phase cells decreased, S phase cells increased. The photosensitization of H_A also makes the cytoplasmic microtubules become thinner and the bright fluorescence near the microtubule center almost disappears. The above experiments show that the photosensitization of H_A on the cultured cells caused cell membrane, cytoskeleton damage, the cell cycle is partially blocked in the S phase, thereby inhibiting cell proliferation.