利什曼病的诊断一般依靠直接检查病原体、淋巴结或骨髓培养及血清学技术,如ELISA、Dot-ELISA、免疫荧光法(IFA)。活动期利什曼病因特异性抗体浓度较高易用血清学方法测出;但是抗体水平较低或接近临界值时,用血清学方法难以判断疾病的不同期。本文作者用Western印迹分析法能较好地解决这个问题。 在西班牙,犬的利什曼病较为流行,约有5％的犬感染利什曼原虫。作者从西班牙的利 Diagnosis of leishmaniasis generally depends on direct examination of pathogens, lymph node or bone marrow culture and serological techniques such as ELISA, Dot-ELISA, and immunofluorescence (IFA). Active leishmaniasis-specific antibodies at higher concentrations are readily detectable by serological methods, but at low or near-critical antibody levels, it is difficult to determine the different stages of the disease by serological methods. The author of this article can better solve this problem by using Western blot analysis. In Spain, leishmaniasis in dogs is more prevalent, with about 5% of dogs infected with Leishmania. Author Lee from Spain