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采用高通量16S rRNA标签测序法,比较了地处北亚热带与暖温带过渡带的宝天曼自然保护区不同林龄与林分类型的土壤细菌群落结构及多样性.结果表明:宝天曼森林土壤细菌以变形菌门(29%)、酸杆菌门(18.5%)、疣微菌门(10%)等为主,共检测到60门1209属,优势属主要有疣微菌门的DA101(6.3%)、酸杆菌门的Acidobacteria-2(5.9%)和Candidatus Solibacter(2.9%)、泉古菌门的Candidatus Nitrososphaera(2.6%)等.不同林龄和林分类型土壤分别具有特有的种属组成及高丰度和低丰度种属.林龄与林分类型都对土壤微生物群落结构影响显著,且林分类型的影响大于林龄.80年林龄的锐齿栎土壤菌群多样性在不同林龄和林分类型中均最低.p H、土壤全氮、有机碳等是不同林龄及林分类型下土壤菌群结构变化的重要影响因子.
Using high-throughput 16S rRNA sequencing, the bacterial community structure and diversity of different ages and forest types in Baotianman Nature Reserve located in the north subtropical zone and warm temperate zone were compared. The results showed that Baotianman The main bacteria in the forest soil were Proteobacteria (29%), Acidobacteria (18.5%), Mycobacterium verrucosa (10%) and so on. A total of 601 1209 genera were detected, (6.3%), Acidobacteria-2 (5.9%) and Candidatus Solibacter (2.9%), Acididobacteriaceae Candidatus Nitrososphaera (2.6%), etc. The soil types with different stand age and stand types have endemic species Is composed of high abundance and low abundance species.Lint age and stand type have a significant impact on soil microbial community structure, and the impact of stand type is greater than the age of the forest.Occupation of 80-year-old Dendrolimus krymsis soil microbial diversity The lowest was in different stand ages and stand types, while p H, soil total nitrogen and organic carbon were the important influencing factors of soil microbial community structure under different stand ages and stand types.